Semen processing for the subfertile stallion
Dickson D Varner et al
Journal of Veterinary Science, Vol 28, no 11 (2008)
“From these data, one can surmise that the EquiPure™ centrifugation procedure considerably improved the seminal quality of this stallion, as measures of sperm-motion characteristics and sperm morphology were enhanced with this procedure”
Use of a Silane-Coated Particle Solution to Enhance the Quality of Ejaculated Semen in Stallions
D.D Varner et al
49th Annual Convention for the American Association fo Equine Practitioners, 2003, New Orleans
“This study demonstrated that use of a proprietary silanated silica particle density gradient (EquiPure™) can improve semen quality in stallions, as determined by measures of both spermatozoal motility and spermatozoal morphology”
Does the microbial flora in the ejaculate affect the freezeability of stallion sperm?
C Ortega-Ferrusola et al
Reprod. Dom Anim 44, (2009) pp 518-522
“It is concluded that the microbial flora of the equine ejaculate may be responsible for some of the sublethal damage experimented by the spermatozoa during cryopreservation”
(Note: EquiPure removes the microbial flora and also increases survival rates after freezing)
Insemination Doses: how low can we go?
S P Brinsko
Theriogenology 66(2006) 543-550
Dept of Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 78843-4475, USA
“…conducted breeding trials with two stallions having per-cycle pregnancy rates of <20%and showed improvement in pregnancy rate after their semen was centrifuged through a discontinous density gradient (EquiPure™) to increase the percentage of progressively, morphologically normal sperm. Hysteroscopic insemination with 100µl of extended semen containing 20 x 106 PMS increased the pregnancy rate from 20 to 35% (7/20) for one stallion and transrectally guided deep-horn insemination of 60-150 x 106 PMS in 250 µl resulted in seven of eight mares (87.5%) becoming pregnant for the other horse”
Advanced Methods for Handling and Preparation of Stallion Semen
Loomis et al
Anim Reprod Sci. 2006 Feb;91(3-4):237-47
Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
“Therefore, advanced methods for processing and selecting stallion semen provide the clinician with valuable tools for handling poor-quality semen or for obtaining spermatozoa for assisted reproduction procedures”
Effective Removal of Equine Arteritis Virus From Stallion Semen
Geraghty, R.M.
Equine Veterinary Journal, Volume 38, Number 3, May 2006 , pp. 224-229(6)
“Results: Virus-free sperm preparations were obtained using the double sperm processing technique. The ‘swim-up’ step is apparently required to ensure complete virus removal.
Conclusions: The double semen processing technique is potentially a useful and simple tool for the removal of EAV from the semen of shedding stallions”
Effect of Density-Gradient Centrifugation on Quality and Recovery Rate of Equine Spermatozoa
A.J Edmont, DD Varner et al.
Anim Reprod Sci. Issues 3-4, 2008 September; page 318
Dept of Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 78843-4475, USA
“The sperm EquiPure™ centrifugation technique has been used successfully with three commercial breeding stallions in 2007”
(test of different tube sizes)
Evaluation of Cryopreserved-thawed Stallion Sperm Before and after Density Gradient Centrifugation with Silane-coated Silica Particles (EquiPure®)
Stoll A, et al.
Theriogenology 70 (2008) pp. 576-591.
“In conclusion separation of frozen-thawed equine sperm via centrifugation through an EquiPure™ density gradient increased total an dprogressive motility, normal morphology, mitochondrial membrane potential, and plasma membrane alteration of the separated sperm population (comared to the initial control)”
